The Comparison of different fatty acids extraction methods of marine organism: a case study of <i>Penaeus monodon</i>

WANG Fu-qiang; WU Ying; CUI Ying

doi:10.12111/j.mes20190116

Volume 38 Issue 1

Feb. 2019

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Chinese Journal of MARINE ENVIRONMENTAL SCIENCE > 2019 > 38(1): 100-105. > DOI: 10.12111/j.mes20190116

WANG Fu-qiang, WU Ying, CUI Ying. The Comparison of different fatty acids extraction methods of marine organism: a case study of Penaeus monodon[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2019, 38(1): 100-105. DOI: 10.12111/j.mes20190116

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The Comparison of different fatty acids extraction methods of marine organism: a case study of Penaeus monodon

State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China

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Received Date: July 24, 2017
Revised Date: November 18, 2017

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Abstract

This paper took Penaeus monodon as the study object to try to optimize fatty acids extraction of marine biological samples by comparing the extracting means and choosing different transfer regents.The results suggested that the method using microwave extraction with n-hexane and methanol (containing 5% HCl) as transfer reagents was the best method.The recovery of this method was higher than 90%.Moreover, the experiment procedure was simplyfied and the extraction time was shortened.Meanwhile, this method had no selective influence on the relative content of individual fatty acids, and could be widely used in the extraction of fatty acids of marine biological samples.
- Penaeus monodon,
- fatty acids,
- microwave extraction,
- ultrasonic extrection,
- transfer reagents

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