Citation: | TIAN Ze, HE Chen, ZHUO Xiao-cun, WU Yi-xin, HUANG Hai-yan, SHI Quan, SONG Gui-xue. Large volume marine DOM isolation and fractionation by DAX-8 and XAD-4 resins-in-tandem and their molecular level characterization by FT-ICR MS[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2021, 40(3): 442-449. DOI: 10.12111/j.mes.20200007 |
Dissolved organic matter (DOM) was separated from a large volume of surface sweater in offshore Qingdao by DAX-8 and XAD-4 resins used in tandem. Four fractions, namely hydrophobic acid (HOA), hydrophilic acid (HIA), hydrophobic neutral (HON), and hydrophilic neutral (HIN) were obtained. A total of 0.3 g of marine DOM was isolated from 200 L seawater with the total dissolved organic carbon (DOC) extraction recovery of 48%. The four DOM fractions were characterized in molecular level by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS).More than 4,000 individual molecules were assigned from HOA and HIA fractions, which have relatively greater O/C ratios and greater degrees of oxidative degradation.More than 1,000 individual formulas were assigned from the neutral fractions which have greater H/C ratios and lower degree of condensation. The nitrogen-containing compounds were relatively enriched in hydrophilic fractions (HIA and HIN). The resins-in-tandem method is capable for seawaters with high salinity and low DOM concentration, and can be used to isolated and fractionate DOM fractions with different polarities from a large volume of seawater. The amount and low ash content of samples can meet the requirements of characterization and biological culture experiments.
[1] |
HANSELL D A. Recalcitrant dissolved organic carbon fractions[J]. Annual Review of Marine Science, 2013, 5: 421-445. doi: 10.1146/annurev-marine-120710-100757
|
[2] |
张耀玲. 近海环境中天然有机质的分离与表征[D]. 上海: 华东师范大学, 2013.
|
[3] |
王立英, 吴丰昌, 黎 文, 等. 水体溶解有机质富集分离方法的研究进展[J]. 地球与环境, 2008, 36(2): 171-178.
|
[4] |
卫丹丹, 王映辉, 许云平. 海水溶解有机质分离富集方法的发展与比较[J]. 海洋环境科学, 2019, 38(6): 977-984. doi: 10.12111/j.mes20190623
|
[5] |
VETTER T A, PERDUE E M, INGALL E, et al. Combining reverse osmosis and electrodialysis for more complete recovery of dissolved organic matter from seawater[J]. Separation and Purification Technology, 2007, 56(3): 383-387. doi: 10.1016/j.seppur.2007.04.012
|
[6] |
MAURICE P A, PULLIN M J, CABANISS S E, et al. A comparison of surface water natural organic matter in raw filtered water samples, XAD, and reverse osmosis isolates[J]. Water Research, 2002, 36(9): 2357-2371. doi: 10.1016/S0043-1354(01)00442-0
|
[7] |
DITTMAR T, KOCH B, HERTKORN N, et al. A simple and efficient method for the Solid-Phase Extraction of Dissolved Organic Matter (SPE-DOM) from seawater[J]. Limnology and Oceanography: Methods, 2008, 6(6): 230-235. doi: 10.4319/lom.2008.6.230
|
[8] |
王立英, 吴丰昌, 张润宇. 应用XAD系列树脂分离和富集天然水体中溶解有机质的研究进展[J]. 地球与环境, 2006, 34(1): 90-96.
|
[9] |
AIKEN G R, MCKNIGHT D M, THORN K A, et al. Isolation of hydrophilic organic acids from water using nonionic macroporous resins[J]. Organic Geochemistry, 1992, 18(4): 567-573. doi: 10.1016/0146-6380(92)90119-I
|
[10] |
LEENHEER J A. Comprehensive approach to preparative isolation and fractionation of dissolved organic carbon from natural waters and wastewaters[J]. Environmental Science & Technology, 1981, 15(5): 578-587.
|
[11] |
GREEN N W, MCINNIS D, HERTKORN N, et al. Suwannee river natural organic matter: isolation of the 2R101N reference sample by reverse osmosis[J]. Environmental Engineering Science, 2015, 32(1): 38-44. doi: 10.1089/ees.2014.0284
|
[12] |
GREEN N W, PERDUE E M, AIKEN G R, et al. An intercomparison of three methods for the large-scale isolation of oceanic dissolved organic matter[J]. Marine Chemistry, 2014, 161: 14-19. doi: 10.1016/j.marchem.2014.01.012
|
[13] |
SHI Q, PAN N, LONG H Y, et al. Characterization of middle-temperature gasification coal tar. Part 3: molecular composition of acidic compounds[J]. Energy & Fuels, 2013, 27(1): 108-117.
|
[14] |
ESTEVES V I, OTERO M, SANTOS E B H, et al. Stable carbon isotope ratios of tandem fractionated humic substances from different water bodies[J]. Organic Geochemistry, 2007, 38(6): 957-966. doi: 10.1016/j.orggeochem.2007.02.006
|
[15] |
BRONK D A. Dynamics of DON[M]//HANSELL D A, CARLSON C A. Biogeochemistry of marine dissolved organic matter. San Diego: Academic Press, 2002: 153–247.
|
[16] |
ANTONY R, GRANNAS A M, WILLOUGHBY A S, et al. Origin and sources of dissolved organic matter in snow on the East Antarctic ice sheet[J]. Environmental Science & Technology, 2014, 48(11): 6151-6159.
|
[17] |
HOCKADAY W C, PURCELL J M, MARSHALL A G, et al. Electrospray and photoionization mass spectrometry for the characterization of organic matter in natural waters: a qualitative assessment[J]. Limnology and Oceanography: Methods, 2009, 7(1): 81-95. doi: 10.4319/lom.2009.7.81
|
[18] |
LU Y H, LI X P, MESFIOUI R, et al. Use of ESI-FTICR-MS to characterize dissolved organic matter in headwater streams draining forest-dominated and pasture-dominated watersheds[J]. PLoS One, 2015, 10(12): e0145639. doi: 10.1371/journal.pone.0145639
|
[19] |
FENG L, XU J Z, KANG S C, et al. Chemical composition of microbe-derived dissolved organic matter in cryoconite in Tibetan plateau glaciers: insights from Fourier transform ion cyclotron resonance mass spectrometry analysis[J]. Environmental Science & Technology, 2016, 50(24): 13215-13223.
|
[20] |
HERTKORN N, BENNER R, FROMMBERGER M, et al. Characterization of a major refractory component of marine dissolved organic matter[J]. Geochimica et Cosmochimica Acta, 2006, 70(12): 2990-3010. doi: 10.1016/j.gca.2006.03.021
|
[21] |
KOCH B P, WITT M, ENGBRODT R, et al. Molecular formulae of marine and terrigenous dissolved organic matter detected by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry[J]. Geochimica et Cosmochimica Acta, 2005, 69(13): 3299-3308. doi: 10.1016/j.gca.2005.02.027
|
[22] |
JONES T P, CHALONER W G. Fossil charcoal, its recognition and palaeoatmospheric significance[J]. Palaeogeography, Palaeoclimatology, Palaeoecology, 1991, 97(1/2): 39-50.
|
[23] |
KOCH B P, DITTMAR T. From mass to structure: an aromaticity index for high-resolution mass data of natural organic matter[J]. Rapid Communications in Mass Spectrometry, 2006, 20(5): 926-932. doi: 10.1002/rcm.2386
|
[24] |
STUBBINS A, SPENCER R G M, CHEN H M, et al. Illuminated darkness: molecular signatures of Congo River dissolved organic matter and its photochemical alteration as revealed by ultrahigh precision mass spectrometry[J]. Limnology and Oceanography, 2010, 55(4): 1467-1477. doi: 10.4319/lo.2010.55.4.1467
|
[25] |
SEIDEL M, YAGER P L, WARD N D, et al. Molecular-level changes of dissolved organic matter along the Amazon River-to-ocean continuum[J]. Marine Chemistry, 2015, 177: 218-231. doi: 10.1016/j.marchem.2015.06.019
|
[1] | JIN Fei, WANG Ying, CONG Yi, ZHANG Mingxing, LI Zhaochuan, LOU Yadi, YAO Ziwei, WANG Juying. Research on environmental priority pollutants in seawater of China by COMMPS and DYNAMEC methods[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2023, 42(6): 892-900. DOI: 10.12111/j.mes.2023-x-0094 |
[2] | WANG Jia-han, LI Zheng-he, HUANG Jin-song, YANG Feng, YANG Xiu-jiu. Simultaneous determination of 50 elements in marine sediments by microwave digestion ICP-MS[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2021, 40(4): 611-618, 624. DOI: 10.12111/j.mes.20200175 |
[3] | LIU Hong-yan, LI Kai-qiang, KANG Bo-lun, QIN Hai-hua. Fe(Ⅲ) reduction and hydrogen production by Fe (Ⅲ)-reducing bacterium Enterococcus sp. ZQ21[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2021, 40(3): 379-383. DOI: 10.12111/j.mes.20200119 |
[4] | WEI Hai-feng, TIAN Shan-chuan, ZHAO Xiao-yi, LIU Chang-fa, ZHOU Ji-ti. Study on the bioaccumulation kinetics of three PAHs by Apostichopus japonicus[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2019, 38(5): 663-668. DOI: 10.12111/j.mes20190503 |
[5] | LIU Yao, SONG Jin-ming, SUN Ling-ling, YU Ying, SUN Xuan. Determination of trace rare earth elements in seawater by ICP-MS with Mg(OH)2 co-precipitation treatment[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2019, 38(2): 303-309. DOI: 10.12111/j.mes20190220 |
[6] | WANG Hong-yu, FENG Rui-qi, LIU Hong-yan. Characterization of cell growth and bioflocculant production by Enterococcus sp.Y025 using macro-algae Laminaria japonica[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2018, 37(3): 338-342. DOI: 10.12111/j.cnki.mes20180304 |
[7] | ZHENG Wen-jing, HAN Yu, QIN Chuan, ZHANG Gui-ling. Continuous underway measurements of sea surface O2/Ar and pCO2 by membrane inlet mass spectrometry[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2016, 35(4): 611-617. DOI: 10.13634/j.cnki.mes20160421 |
[8] | QIAO Ling, ZHEN Yu, MI Tie-Zhu. Review of the brown tides caused by Aureococcus anophagefferens[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2016, 35(3): 473-480. DOI: 10.13634/j.cnki.mes20160324 |
[9] | LI Lei, JIANG Mei, SHEN Xin-qiang, WANG Yun-long, WU Qing-yuan, NIU Jun-xiang, XU Gao-peng. Effects of Cr(VI) on the activities of SOD,concentration of MDA and MTs inhepatopancreas and gilltsissue of Portunus trituberculatus[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2015, 34(6): 838-843. DOI: 10.13634/j.cnki.mes.2015.06.007 |
[10] | WANG Li-jun, LI Wen-jun, HE Guang-kai, LIU Guang-yuan. Application of microwave-digestion-ICP-MS determination and uncertainty evaluation in a trace elements intercomparision exercise of marine sediment[J]. Chinese Journal of MARINE ENVIRONMENTAL SCIENCE, 2015, 34(3): 451-456. DOI: 10.13634/j.cnki.mes.2015.03.022 |