Abstract: Based on the analysis and identification of 10 sampling sections obtained in biological samples in the intertidal zone of Liaodong Gulf in August 2014, adopt the species diversity index, species abundance index and species evenness index to analyze the diversity of species of the macrobenthos in the intertidal zone; use community clustering, MDS ranking and ABC curve to analyze the community structure characteristics of the macrobenthos in the intertidal zone, and further analyze and expound the distribution reason of community characteristics of macrobenthos. The survey finds a total of 85 species of macrobenthos, including 50 mollusks, 20 arthropods, 8 annelids, 2 coelenterates, 1 echinoderm and 4 kinds of other animals. The average population density and biomass are 321.07/m2and 62.47 g/m2. The data analysis shows that the macrobenthos community in each fracture surface of Liaodong Gulf is disturbed to different extent, and the community characteristics distribution is mainly influenced by the substrate, habitat, season and man-made interference.