Abstract: Antibiotic resistance bacteria and related resistance genes in five mariculture areas of Shandong were detected,and in both five samples it was found that the percentage of tetracycline-,Sulfonamides- and -lactam-resistant bacteria were significantly (p0.05) higher than those chloromycetin- and quinolone-resistant bacteria.The abundance of 15 antimicrobial resistant genes was determined by RT-PCR methods.It was obtained that the abundance of sulfonamides-(sul,dfra/16S rRNA=10-6~10-2),quinolone-(qnr/16S rRNA=10-6~10-2),and tetracycline-resistance genes (tet/16S rRNA=10-7~10-2) has no significant differences among the five water samples,but the abundance of chloramphenicol-resistant genes (cata,cmle/16S rRNA=10-8~10-2) has significant differences among the five water samples and the abundance of the cata1 and cmle1 has significant correction (p0.05) with the percentage of chloramphenicol-resistant bacteria.All the results indicated the emergence pollution of antimicrobial resistant bacteria and related resistance genes in mariculture environments of Shandong,China.