Abstract: In this research, the surface sediment samples were collected from the Muping Marine Ranch and its adjacent area in November 2017.The exchange fluxes of nutrients across the sediment-water interface were estimated by a laboratory incubation method.In addition, the effects of dissolved oxygen and temperature on the fluxes were also studied.The results indicated that the average exchange fluxes of NO₂-N, NO₃-N, NH₄-N and PO₄-P across sediment-water interface were 1.01, -181.91, -268.41 and -45.69 μmol/(m²·d), respectively.The sediments acted as a "source" of NO₂-N and a "sink" of NO₃-N, NH₄-N and PO₄-P.Both dissolved oxygen and temperature had obvious influence on the fluxes.The results showed that the fluxes of NO₂-N, NH₄-N and PO₄-P were higher in the aerobic situation than in the anoxic situation, while that of NO₃-N showed the opposite trend; the fluxes of NO₂-N, NO₃-N and NH₄-N were higher at a higher temperature, but that of PO₄-P was opposite to them.