固定化硫氧化菌对海水养殖生境中硫化物的控制效果研究

Control of sulfide in aquaculture water by immobilized sulfur-oxidizing bacteria

  • 摘要: 海水养殖生境中由于有机质积累以及缺氧条件导致硫化物大量产生,严重危及养殖环境健康。为此,创建海水养殖系统,以沸石为载体吸附固定化硫氧化菌株,通过定位布放固定化硫氧化菌袋,探究其对生境中硫化物控制效果及其影响因素。结果表明,固定化菌剂中活菌含量为1.9×107 CFU/g,对硫化物的控制效果受环境温度、pH、菌剂投加量的影响较大,对温度及pH变化有更好的耐受性;在温度为30℃、pH为7.0、150 r/min的实验条件下,对硫化物的最大去除速率为8.3 mg/(g·h·L)。在海水养殖环境中,将沸石固定化菌剂平铺于土工布袋内,将布袋平铺于养殖系统沉积物-水界面,覆盖率2.5%,固定化布袋菌剂能够显著抑制环境中硫化物的产生,在缺氧条件下7 d内对硫化物的控制率达到99%。本研究表明定点布放硫氧化菌袋能够很好的控制养殖水体中硫化物,为其在海水养殖系统中的应用提供了技术支撑。

     

    Abstract: In marine aquaculture habitats, large amounts of sulfides are produced due to the accumulation of organic matter and anoxic conditions, which seriously endanger the health of the aquaculture environment.For this purpose, a marine aquaculture system was established to adsorb immobilized sulfur-oxidizing strains with zeolite as a carrier, and the immobilized sulfur oxidizing bacteria bag was positioned and positioned to explore its control effect on the sulfides in the habitat and its influencing factors.The results showed that the viable bacteria content in the immobilized microbial agent was 1.9×107 CFU/g, and the control effect on sulfides was greatly affected by the ambient temperature, pH, and dosage of the microbial inoculum, and the resistance to temperature and pH changes was better.The maximum removal rate of sulphide under experimental conditions of 30℃, pH 7.0, and 150 r/min was 8.3 mg/(g·h·L).In aquaculture environment, the zeolite immobilized microbial inoculum was laid in a geotextile bag and the cloth bag was laid flat on the central sediment-water interface of the culture system.The coverage was 2.5%.It was found that the immobilized microbial inoculant can significantly inhibit the vulcanization in the environment.The production of the substance controlled 99% of sulfides within 7 days under anoxic conditions.This study shows that the deployment of sulfur oxidizing bacteria at fixed points can control sulphides in aquaculture waters and provide data support for its application in marine aquaculture systems.

     

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