Abstract: After the oil spill, amphipods disappeared rapidly and showed very high initial mortalities. The study was investigated the acute toxic effects of crude oil on the Corophium acherusicum (Crustacean, Amphipod) by 96 h acute toxicity test．The data of acute toxicity of WAF and CEWAF to marine organisms of different trophic levels were screened and collected. The species sensitivity distribution (SSD) method was used to evaluate the sensitivity of C.acherusicum to crude oil. The results showed that WAF and CEWAF 96 h LC₅₀ were 4.11 mg/L, 0.85 mg/L respectively. The calculated results of the cumulative probability showed that the C.acherusicum to WAF and CEWAF were lower than 30%. In summary, C.acherusicum was relatively sensitive to crude oil.