Abstract: Marine dinoflagellate Prorocentrum lima producing diarrhetic shellfish poison were mass cultured and crushed by ultrasonic, followed by extracting with methanol for okadaic acid (OA) and dinophysis toxin-1(DTX1). OA and DTX1 with high purity were obtained by a preparative high performance liquid chromatographic (HPLC) method after enrichment and purification with macroporous adsorption resin HP20, which was used for the enrichment of extracellular toxin secreted in the sea water. The two compounds were identified by nuclear magnetic resonance hydrogen spectrum (1H-NMR) and high-resolution mass spectrometry (HRMS). The purity of OA and DTX1 was identified by quantitative nuclear magnetic to be 99.39% and 99.26% respectively, meeting the requirements for preparation of standard material.