Abstract: The effects of petroleum hydrocarbons on ingestion and metabolism of Calanus sinicus were studied. The results showed that petroleum hydrocarbons had significant effects on filtering rate, ingestion rate, respiratory rate and ammonium excretion rate of C. sinicus (p<0.05). Ammonium excretion rate was significantly different from every concentration groups (p<0.05), which was the most sensitive to the changes of petroleum hydrocarbon concentration. There were no significant differences in filtering rate, ingestion rate, respiratory rate and ammonium excretion rate between 0.051 mg/L, 0.103 mg/L, 0.215 mg/L concentration groups and the control group (p>0.05) after 14 days of recovering culture. The filtering rate and ingestion rate of 0.43 mg/L and 0.86 mg/L concentration groups were significantly differences from the control group (p<0.05), and no significant differences in respiratory rate and ammonium excretion rate (p>0.05). In the control group and low concentration groups, protein metabolism was dominant metabolism styles, the O∶N ratio increased when exposed to 0.43 mg/L and 0.86 mg/L concentration groups, carbohydrates or fats began to participate in metabolism. After 14 days of recovering culture, the metabolic was still based on protein in all concentration groups.