Abstract：We studied the change of the antioxidant system of Prorocentrum donghaiense during lysing process treated with Halomona Sp.DH-e cell-free filtrate and the acute toxicity test of fermentation products to explore the inhibitory mechanism of DH-e on the P.donghaiense and acute toxicity of DH-e fermentation products.The results showed that the reactive oxygen species (ROS) metabolism of algae cell had an increasing trend after treated with DH-e aseptic filtrate.Then the self-protection mechanism of algal cells was started.Furthermore,of the activities of antioxidant enzymes,such as superoxide dismutase (SOD) and catalase (CAT),and Malodialdehyde (MDA) content both were increased.We found that the semi-lethal concentration was LC50 > 1 00 mg/L when the zebrafish treated with fermentation products for 96h.The mortality rate of zebrafish effected by fermentation maximum dose (5 000 mg/kg·bw) was 0% in 14 d (LD50 > 5 000 mg/kg·bw).These results revealed that the fermentation is low-toxic.
WU Pei-feng,HAN Guang-yao,XIE Li-ling et al. Effects of the Halomona Sp.DH-e aseptic filtrate on the antioxidant enzyme system of Prorocentrum donghaiense and its acute toxicity[J]. Marine Environmental Science, 2018, 37(2): 228-232.
LI M,HU C W,ZHU Q,et al.Copper and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in the microalga Pavlova viridis (Prymnesiophyceae)[J].Chemosphere,2006,62(4):565-572.
[5]
AFFENZELLER M J,DAREHSHOURI A,ANDOSCH A,et al.Salt stress-induced cell death inthe unicellular green alga Micrasterias denticulata[J].Journal of Experimental Botany,2009,60(3):939-954.
LIU I,LAWTON L A,CORNISH B,et al.Mechanistic and toxicity studies of the photocatalytic oxidation ofmicrocystin-LR[J].Journal of Photochemistry and Photobiology A:Chemistry,2002,148(1/2/3):349-354.
[8]
ZHU L Y,HUANG Y,LIU G X.Using DNA damage to monitor water environment[J].Chinese Journal of Oceanology and Limnology,2005,23(3):340-348.
BOURAOUI Z,BANNI M,GHEDIRA J,et al.Evaluation of enzymatic biomarkers andlipoperoxidation level in Hediste diversicolor exposed to copper and benzo[a]pyrene[J].Ecotoxicology and Environmental Safety,2009,72(7):1893-1898.
WANG Q,SU M,ZHU W,et al.Growth inhibition of Microcystis aeruginosa by white-rot fungus Lopharia spadicea[J].Water Science and Technology,2010,62(2):317-323.
[14]
BANERJEE B D,SETH V,BHATTACHARYA A,et al.Biochemical effects of some pesticides on lipid peroxidation and free-radical scavengers[J].Toxicology Letters,1999,107(1/2/3):33-47.
