Abstract：A large application of nano-materials increases their risk entering the marine environment.The primary purpose of this study is to explore the toxic effects and mechanisms of nano-TiO2,which is widely applied in industry and commercial products.Algal growth inhibition test was carried out,and chlorophyll concentration and lipid peroxidation level were measured using Skeletonema costatum and Dunaliella salina as model organisms.Moreover,adsorption of nano-TiO2 on microalgae was observed by SEM technique.The settlement of nano-TiO2 particles in experimental medium with 24 h was also characterized.Results showed that both growth and photosynthesis of S.costatum and D.salina were inhibited by nano-TiO2.Toxic effects of nano-TiO2 on S.costatum were similar to those of D.salina.Adsorption of nano-TiO2 and oxidative damages resulting from interactions between nano-TiO2 and microalgae could inhibit growth and photosynthesis of microalgae,which were the main reasons for toxic effects of nano-TiO2.The settlement of nano-TiO2 particles in experiment medium caused the decrease of suspension concentration which had an influence on results.
ZHANG Cai,CHEN Xiao-hua,TAN Li-ju et al. Research on toxic effects of nano-TiO2 on Skeletonema costatum and Dunaliella salina[J]. Marine Environmental Science, 2018, 37(2): 215-220,227.
PICCINNO F,GOTTSCHALK F,SEEGER S,et al.Industrial production quantities and uses of ten engineered nanomaterials in Europe and the world[J].Journal of Nanoparticle Research,2012,14:1109.
[3]
BERNIER M C,EL KIRAT K,BESSE M,et al.Preosteoblasts and fibroblasts respond differently to anatase titanium dioxide nanoparticles:a cytotoxicity and inflammation study[J].Colloids and Surfaces B:Biointerfaces,2012,90:68-74.
[4]
NASU A,OTSUBO Y.Rheology and UV-protecting properties of complex suspensions of titanium dioxides and zinc oxides[J].Journal of Colloid and Interface Science,2007,310(2):617-623.
[5]
GOTTSCHALK F,SONDERER T,SCHOLZ R W,et al.Modeled environmental concentrations of engineered nanomaterials (TiO2,ZnO,Ag,CNT,fullerenes) for different regions[J].Environmental Science & Technology,2009,43(24):9216-9222.
[6]
DANOVARO R,BONGIORNI L,CORINALDESI C,et al.Sunscreens cause coral bleaching by promoting viral infections[J].Environmental Health Perspectives,2008,116(4):441-447.
[7]
WANG Z,WANG S,PEIJNENBURG W J G M.Prediction of joint algal toxicity of nano-CeO2/nano-TiO2 and florfenicol:Independent action surpasses concentration addition[J].Chemosphere,2016,156:8-13.
[8]
ADACHI K,YAMADA N,YAMAMOTO K,et al.In vivo effect of industrial titanium dioxide nanoparticles experimentally exposed to hairless rat skin[J].Nanotoxicology,2010,4(3):296-306.
[9]
WARHERT D B,HOKE R A,FINLAY C,et al.Development of a base set of toxicity tests using ultrafine TiO2 particles as a component of nanoparticle risk management[J].Toxicology Letters,2007,171(3):99-110.
[10]
CLÉMENT L,HUREL C,MARMIER N.Toxicity of TiO2 nanoparticles to cladocerans,algae,rotifers and plants-effects of size and crystalline structure[J].Chemosphere,2013,90(3):1083-1090.
[11]
ISWARYA V,BHUVANESHWARI M,Alex S A,et al.Combined toxicity of two crystalline phases (anatase and rutile) of Titania nanoparticles towards freshwater microalgae:Chlorella sp[J].Aquatic Toxicology,2015,161:154-169.
WANG Y X,ZHU X S,LAO Y M,et al.TiO2 nanoparticles in the marine environment:Physical effects responsible for the toxicity on algae Phaeodactylum tricornutum[J].Science of the Total Environment,2016,565:818-826.
[16]
LIN D H,JI J,LONG Z F,et al.The influence of dissolved and surface-bound humic acid on the toxicity of TiO2 nanoparticles to Chlorella sp[J].Water Research,2012,46(14):4477-4487.
[17]
ARAVANTINOU A F,TSARPALI V,DAILIANIS S,et al.Effect of cultivation media on the toxicity of ZnO nanoparticles to freshwater and marine microalgae[J].Ecotoxicology and Environmental Safety,2015,114:109-116.
[18]
侯东颖.纳米二氧化钛胁迫对普生轮藻的毒性效应[D].太原:山西大学,2012.
[19]
ARUOJA V,DUBOURGUIER H C,KASEMETS K,et al.Toxicity of nanoparticles of CuO,ZnO and TiO2 to microalgae Pseudokirchneriella subcapitata[J].Science of the Total Environment,2009,407(4):1461-1468.
